This dataset includes 3 conditions: unwounded diabetic skin, diabetic non-healing wounds and diabetic healing wounds. For this analysis, we will compare non-healing vs healing diabetic wounds.
WOUND HEALING
Data Analysis Instructions
1
Access the data on the GEO2R platform. The link opens in a new window.
GEO2R GSE1344312
Click “Define groups” in the title bar.
Type in the name of the first group, “healer”, and press enter.
Type in the name of the second group, “non-healer”, and press enter.
3
Select all samples labeled “Healer” in the Healer_or_nonhealer column. You can click and drag over multiple rows.
Click the group name “healer” that you previously created, to link the samples to the group.Image
4
Repeat Step 3, now selecting all “Non-Healer” samples in the Healer_or_nonhealer column. You may have to scroll down the page.
Click the “non-healer” group name to link those samples to that group.Image
5
Click the “Analyze” button at the bottom of the page.
6
After the analysis is complete, you should see a volcano, mean difference, UMAP, Venn, Box and other plots informing QC and differential expression results as well as a table of differentially expressed genes:
Image
7
To plot the expression of your gene of interest across samples:Click on the “Profile graph” tabType in your gene name and press “Set”.
